Vitamin E analogs by HPLC directly from biodiesel


The determination of minor components, such as sterols and tocopherols in oil (vegetable oils, bio-diesel) is usually carried out by gas chromatography after prior treatment. This includes the concentration of the components, separation of the interfering matrix components and derivatization of the analyte prior to the actual measurement.

The current technology is based on a fractionation by HPLC, Derivatization of tocopherols (to silyl derivatives) followed by gas chromatographic determination. The direct provision of these components by means of HPLC is possible for the tocopherols by UV and fluorescence detection. This results in the ability to detect all analytes by means of highly sensitive fluorescence detection even from the native oil.

For the separation of tocopherols typically normal phase HPLC is clearly preferred because of their ability to completely separate the isomers. This application allows the determination of all vitamin E analogues using RP chromatography, so that eliminates the difficult handling with normal phase and a much higher stability of the determination is made​ ​.

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